Mutation

As with any other protein, rhodopsin has a few mutations.  The most common and well studied is retinal pigmentosa.  Retinal pigmentosa is chareacterised by the loss of function followed by slow progressive degeneration of  the peripheral retina.  There are a lot of various reasons for this disorder.  The most common ones occur as one of 13 point mutations in 12 amino acid positions in opsin and can be classified into two subclasses. 

Class 1:

Phe 45--> Leu

Glu 314 --> stop codon

Pro 347--> Leu

The mutations from this class resemble the wild type rhodopsin in yield, regeneratability, and plasma membrane location.

Class 2:

Thr17--> Met

Pro 23--> His

Thr 58--> Arg

Val 87--> Asp

Gly 89--> Asp

Gly 106--> Trp

Arg 135--> Leu

Arg 135-->Trp

Try 178--> Cys

Arg 190--> Gly

The mutations from this class have low opsin yield, variability in regeneration or no regeneration at all, is found mostly in the endoplasmic reticulum indicating inefficient transportation to the plasma membrane.

Another mutation is Stationary night blindness--This mutation is caused by the lack of enough Vitamin A to make the chromophore and regenerate rhodopsin.  this causes a delay in the regeneration of rhodopsin because there is not availible 11-cis retinal to bind to free opsin.  This is deficiency causes an increase in rod threshold.  This implies that a larger amount of photons must be absorbed for the rhodopsin retinal component to change conformation.  Thus, in low light areas such as during the night, a person with the condition will not be able to see because the signal is not being relayed to the brain.  Hence, the name night blindness.